Journal: Molecular Neurodegeneration

Article Title: BACE1 regulates expression of Clusterin in astrocytes for enhancing clearance of β-amyloid peptides

doi: 10.1186/s13024-023-00611-w

Figure Lengend Snippet: BACE1 deficiency enhances P38, ERK1/2, and cJun phosphorylation. A Western blot of BACE1-null and WT primary astrocytes lysates. Images indicate major bands for pJun, total Jun, pP38, total P38, pERK1/2, pJNK and Calnexin. B Quantification of pJun/total cJun, pP38/total p38, and pERK1/2 band intensity normalized to Calnexin ( N = 3, * p -value < 0.05, One-way ANOVA with Tukey post testing comparing between samples)

Article Snippet: Since insulin is already present in the G-5 supplemented astrocyte media, we inhibited insulin signaling by using BMS-754807 (SelleckChem), a potent IR tyrosine kinase phosphorylation inhibitor (Fig. C and D).

Techniques: Phospho-proteomics, Western Blot